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Detection of Adulteration in Italian Mozzarella


Considering the importance of monitoring adulterations of genuine cheeses in thedairy industry, a polymerase chain reaction–based method was developed to detect bovine-specific mitochondrial DNA sequence in Italian water buffalo Mozzarella cheese.
DNA was isolated from cheese matrix and governing liquid by organic extractions and kitpurifications. Amplifications of a 134-bp fragment were performed with a bovine–specificset of primers designed on the sequence alignment of bovine and buffalo mitochondrialcytochrome oxidase subunit I. The specificity of the primers was tested using DNA from the blood of two species (water buffalo and bovine), which are present together in adulterated
Italian Mozzarella cheese.

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